Toward the construction of a molecular map of cassava ( Manihot esculenta Crantz): comparison of restriction enzymes and probe sources in detecting RFLPs
Identifieur interne : 004531 ( Main/Exploration ); précédent : 004530; suivant : 004532Toward the construction of a molecular map of cassava ( Manihot esculenta Crantz): comparison of restriction enzymes and probe sources in detecting RFLPs
Auteurs : Fernando Angel [Colombie] ; Diana I. Arias [Colombie] ; Joseph Tohme [Colombie] ; Carlos Iglesias [Colombie] ; William M. Roca [Colombie]Source :
- Journal of Biotechnology [ 0168-1656 ] ; 1993.
English descriptors
- Teeft :
- Aesculifolia, Bamhi, Biotechnology research unit, Cassava, Cassava cultivars, Ciat, Clone, Different restriction enzymes, Ecori, Ecorv, Enzyme, First step, Genet, Genetics, Genomic, Genomic probes, Genotype, Highest polymorphism, Hindlii, Hpali, Manihot, Mccouch, More polymorphism, Nucleic acids, Plasmid, Polymorphic, Polymorphic probes, Polymorphism, Probe, Psti, Psti genomic probes, Restriction, Restriction endonucleases, Restriction enzyme, Restriction enzymes, Restriction fragment length polymorphisms, Rflp, Rflps, Southern blots, Tanksley, Wild manihot species, Wild species, Xbai, Xbai genomic probes.
Abstract
Abstract: The construction of a detailed genetic map of cassava (Manihot esculenta Crantz), classified as a tetraploid species, depends on the ability of cloned sequences to detect polymorphisms. As a first step in developing this map, 200 cloned nuclear sequences generated with different restriction enzymes were hybridized to total digested DNA from eleven cultivated lines and one wild Manihot species, M. aesculifolia. Polymorphism was detected less frequently with both BamHI and EcoRI genomic probes than with PstI, HindIII and XbaI genomic probes. DNA digested with HpaII, DraI and TaqI displayed less polymorphism, whereas DNA digested with EcoRI and EcoRV displayed more polymorphism like that found in lettuce, rice and tomato (Landry et al., 1987; McCouch et al., 1988; Miller and Tanksley, 1990). Four-cutter restriction enzymes displayed less frequency of polymorphism when compared with six-cutter restriction enzymes. Polymorphism displayed by DraI was extremely low, indicating that regions rich in adenine and thymine may not be hot spots for mutation in cassava. Polymorphism detected between cultivated genotypes and M. aesculifolia was dramatically higher than that found among cultivated genotypes.
Url:
DOI: 10.1016/0168-1656(93)90140-I
Affiliations:
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Roca</name><affiliation wicri:level="1"><country xml:lang="fr">Colombie</country><wicri:regionArea>Correspondence to: William M. Roca, Biotechnology Research Unit, Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Colombie</country><wicri:regionArea>Biotechnology Research Unit, Centro Internacional de Agricultura Tropical (CIAT), Cali</wicri:regionArea><wicri:noRegion>Cali</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Biotechnology</title><title level="j" type="abbrev">BIOTEC</title><idno type="ISSN">0168-1656</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">31</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="103">103</biblScope><biblScope unit="page" to="113">113</biblScope></imprint><idno type="ISSN">0168-1656</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-1656</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aesculifolia</term><term>Bamhi</term><term>Biotechnology research unit</term><term>Cassava</term><term>Cassava cultivars</term><term>Ciat</term><term>Clone</term><term>Different restriction enzymes</term><term>Ecori</term><term>Ecorv</term><term>Enzyme</term><term>First step</term><term>Genet</term><term>Genetics</term><term>Genomic</term><term>Genomic probes</term><term>Genotype</term><term>Highest polymorphism</term><term>Hindlii</term><term>Hpali</term><term>Manihot</term><term>Mccouch</term><term>More polymorphism</term><term>Nucleic acids</term><term>Plasmid</term><term>Polymorphic</term><term>Polymorphic probes</term><term>Polymorphism</term><term>Probe</term><term>Psti</term><term>Psti genomic probes</term><term>Restriction</term><term>Restriction endonucleases</term><term>Restriction enzyme</term><term>Restriction enzymes</term><term>Restriction fragment length polymorphisms</term><term>Rflp</term><term>Rflps</term><term>Southern blots</term><term>Tanksley</term><term>Wild manihot species</term><term>Wild species</term><term>Xbai</term><term>Xbai genomic probes</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The construction of a detailed genetic map of cassava (Manihot esculenta Crantz), classified as a tetraploid species, depends on the ability of cloned sequences to detect polymorphisms. As a first step in developing this map, 200 cloned nuclear sequences generated with different restriction enzymes were hybridized to total digested DNA from eleven cultivated lines and one wild Manihot species, M. aesculifolia. Polymorphism was detected less frequently with both BamHI and EcoRI genomic probes than with PstI, HindIII and XbaI genomic probes. DNA digested with HpaII, DraI and TaqI displayed less polymorphism, whereas DNA digested with EcoRI and EcoRV displayed more polymorphism like that found in lettuce, rice and tomato (Landry et al., 1987; McCouch et al., 1988; Miller and Tanksley, 1990). Four-cutter restriction enzymes displayed less frequency of polymorphism when compared with six-cutter restriction enzymes. Polymorphism displayed by DraI was extremely low, indicating that regions rich in adenine and thymine may not be hot spots for mutation in cassava. Polymorphism detected between cultivated genotypes and M. aesculifolia was dramatically higher than that found among cultivated genotypes.</div></front></TEI><affiliations><list><country><li>Colombie</li></country></list><tree><country name="Colombie"><noRegion><name sortKey="Angel, Fernando" sort="Angel, Fernando" uniqKey="Angel F" first="Fernando" last="Angel">Fernando Angel</name></noRegion><name sortKey="Arias, Diana I" sort="Arias, Diana I" uniqKey="Arias D" first="Diana I." last="Arias">Diana I. Arias</name><name sortKey="Iglesias, Carlos" sort="Iglesias, Carlos" uniqKey="Iglesias C" first="Carlos" last="Iglesias">Carlos Iglesias</name><name sortKey="Roca, William M" sort="Roca, William M" uniqKey="Roca W" first="William M." last="Roca">William M. Roca</name><name sortKey="Roca, William M" sort="Roca, William M" uniqKey="Roca W" first="William M." last="Roca">William M. Roca</name><name sortKey="Tohme, Joseph" sort="Tohme, Joseph" uniqKey="Tohme J" first="Joseph" last="Tohme">Joseph Tohme</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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